{"id":1759,"date":"2024-09-05T19:26:19","date_gmt":"2024-09-05T11:26:19","guid":{"rendered":"http:\/\/www.hngenetics.org\/?p=1759"},"modified":"2024-09-05T19:26:21","modified_gmt":"2024-09-05T11:26:21","slug":"%e5%88%98%e4%bd%b3-%e6%9d%8e%e5%ae%b6%e5%a4%a7%e5%90%88%e4%bd%9c%e5%8f%91%e7%8e%b0%e6%9d%86%e7%8a%b6%e7%97%85%e6%af%92%e6%84%9f%e6%9f%93%e5%93%ba%e4%b9%b3%e5%8a%a8%e7%89%a9%e7%9a%84%e5%ae%bf%e4%b8%bb","status":"publish","type":"post","link":"http:\/\/www.hngenetics.org\/?p=1759","title":{"rendered":"\u5218\u4f73\/\u674e\u5bb6\u5927\u5408\u4f5c\u53d1\u73b0\u6746\u72b6\u75c5\u6bd2\u611f\u67d3\u54fa\u4e73\u52a8\u7269\u7684\u5bbf\u4e3b\u56e0\u5b50"},"content":{"rendered":"\n<p>\u8fd1\u65e5\uff0c\u4e0a\u6d77\u79d1\u6280\u5927\u5b66\u514d\u75ab\u5316\u5b66\u7814\u7a76\u6240\u5218\u4f73\u8bfe\u9898\u7ec4\u4e0e\u4e2d\u5357\u5927\u5b66\u751f\u547d\u79d1\u5b66\u5b66\u9662\u674e\u5bb6\u5927\u8bfe\u9898\u7ec4\u5408\u4f5c\uff0c\u5229\u7528\u56e2\u961f\u5f00\u53d1\u7684<a><\/a><a>\u819c\u86cb\u767d\u7ec4<\/a>CRISPR\/sgRNA\u6587\u5e93\u9996\u6b21\u9274\u5b9a\u4e86<a><\/a><a>\u6746\u72b6\u75c5\u6bd2<\/a>AcMNPV\u611f\u67d3\u54fa\u4e73\u52a8\u7269\u7ec6\u80de\u7684\u91cd\u8981\u5bbf\u4e3b\u56e0\u5b50EXTL3\u548cNPC1\uff0c\u9610\u660e\u4e86EXTL3\u548cNPC1\u5728\u6746\u72b6\u75c5\u6bd2\u7c98\u9644\u3001\u8fdb\u5165\u53ca\u5185\u4f53\u9003\u9038\u8fc7\u7a0b\u4e2d\u7684\u529f\u80fd\u3002\u8be5\u7814\u7a76\u6210\u679c\u4ee5\u201cEXTL3 and NPC1 are mammalian host factors for <em>Autographa californica<\/em> multiple nucleopolyhedrovirus infection\u201d\uff08EXTL3\u548cNPC1\u662fAcMNPV\u75c5\u6bd2\u611f\u67d3\u54fa\u4e73\u52a8\u7269\u7684\u5bbf\u4e3b\u56e0\u5b50\uff09\u4e3a\u9898\uff0c\u4e8e9\u67084\u65e5\u5728\u7ebf\u53d1\u8868\u5728<em>Nature Communications<\/em>\uff08\u81ea\u7136\u901a\u8baf\uff09\u4e0a\u3002<\/p>\n\n\n\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"444\" src=\"http:\/\/www.hngenetics.org\/wp-content\/uploads\/2024\/09\/\u56fe\u72471-1024x444.png\" alt=\"\" class=\"wp-image-1760\" srcset=\"http:\/\/www.hngenetics.org\/wp-content\/uploads\/2024\/09\/\u56fe\u72471-1024x444.png 1024w, http:\/\/www.hngenetics.org\/wp-content\/uploads\/2024\/09\/\u56fe\u72471-300x130.png 300w, http:\/\/www.hngenetics.org\/wp-content\/uploads\/2024\/09\/\u56fe\u72471-768x333.png 768w, http:\/\/www.hngenetics.org\/wp-content\/uploads\/2024\/09\/\u56fe\u72471.png 1107w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n\n\n\n<p>\u6746\u72b6\u75c5\u6bd2\u5728\u79d1\u7814\u5b9e\u9a8c\u5ba4\u548c\u5de5\u4e1a\u754c\u90fd\u6709\u5e7f\u6cdb\u7684\u5e94\u7528\uff0c\u53ef\u4f5c\u4e3a\u8f7d\u4f53\u8f6c\u5bfc\u5916\u6e90\u57fa\u56e0\u8fdb\u5165\u6606\u866b\u7ec6\u80de\u8fdb\u884c\u86cb\u767d\u8d28\u8868\u8fbe\u3002\u9664\u4e86\u8f6c\u5bfc\u57fa\u56e0\u8fdb\u5165\u6606\u866b\u7ec6\u80de\uff0c\u6746\u72b6\u75c5\u6bd2\u4e5f\u88ab\u5e7f\u6cdb\u7528\u4e8e\u54fa\u4e73\u52a8\u7269\u7cfb\u7edf\u7684\u57fa\u56e0\u8f6c\u5bfc\u3002\u7531\u4e8e\u6746\u72b6\u75c5\u6bd2\u5177\u6709\u8f83\u5927\u7684\u5305\u88c5\u80fd\u529b\u4ee5\u53ca\u5728\u54fa\u4e73\u52a8\u7269\u4f53\u5185\u4e0d\u590d\u5236\u3001\u4e0d\u6574\u5408\u7684\u7279\u6027\uff0c\u56e0\u6b64\u6746\u72b6\u75c5\u6bd2\u88ab\u8ba4\u4e3a\u662f\u4e00\u79cd\u6781\u5177\u53d1\u5c55\u524d\u666f\u7684\u57fa\u56e0\u6cbb\u7597\u8f7d\u4f53\uff0c\u4f46\u6746\u72b6\u75c5\u6bd2\u5728\u54fa\u4e73\u52a8\u7269\u7ec6\u80de\u4e2d\u7684\u8f6c\u5bfc\u673a\u5236\u5c1a\u4e0d\u6e05\u695a\u3002<\/p>\n\n\n\n<p>\u672c\u7814\u7a76\u4e2d\uff0c\u7814\u7a76\u4eba\u5458\u5229\u7528\u819c\u86cb\u767d\u7ec4CRISPR\/sgRNA\u6587\u5e93\uff08SfCRISPR\uff09\u8fdb\u884c\u4e86\u4e24\u8f6e\u7b5b\u9009\uff0c\u786e\u5b9a\u4e86\u5305\u62ec\u5916\u6ccc\u7d20\u6837\u7cd6\u57fa\u8f6c\u79fb\u91763\uff08EXTL3\uff09\u548cNPC\u7ec6\u80de\u5185\u80c6\u56fa\u9187\u8f6c\u8fd0\u86cb\u767d1\uff08NPC1\uff09\u5728\u5185\u7684\u51e0\u79cd\u6746\u72b6\u75c5\u6bd2\u5019\u9009\u5bbf\u4e3b\u56e0\u5b50\u3002\u901a\u8fc7\u673a\u5236\u7814\u7a76\u53d1\u73b0\uff0cEXTL3\u53c2\u4e0e\u786b\u9178\u4e59\u9170\u809d\u7d20\u7684\u751f\u7269\u5408\u6210\u4ecb\u5bfc\u6746\u72b6\u75c5\u6bd2\u7684\u7c98\u9644\u548c\u8fdb\u5165\uff0cNPC1\u5219\u662f\u4ecb\u5bfc\u6746\u72b6\u75c5\u6bd2\u4e0e\u5bbf\u4e3b\u7684\u819c\u878d\u5408\u8fc7\u7a0b\u4ee5\u53ca\u540e\u7eed\u7684\u5185\u4f53\u9003\u9038\u8fc7\u7a0b\u3002\u5728\u4f53\u5185\u5b9e\u9a8c\u4e2d\uff0c\u7814\u7a76\u4eba\u5458\u53d1\u73b0\u6572\u4f4eNpc1\u57fa\u56e0\u7684\u8868\u8fbe\u53ef\u4ee5\u663e\u8457\u964d\u4f4e\u6746\u72b6\u75c5\u6bd2\u5728\u5c0f\u9f20\u809d\u810f\u4e2d\u7684\u8f6c\u5bfc\u6548\u7387\u3002\u8be5\u7814\u7a76\u9996\u6b21\u7cfb\u7edf\u9274\u5b9a\u4e86\u6746\u72b6\u75c5\u6bd2\u5728\u54fa\u4e73\u52a8\u7269\u7ec6\u80de\u4e2d\u7684\u91cd\u8981\u5bbf\u4e3b\u56e0\u5b50\uff0c\u5bf9\u5176\u5e94\u7528\u63d0\u4f9b\u4e86\u7406\u8bba\u4f9d\u636e\u3002<\/p>\n\n\n\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"524\" src=\"http:\/\/www.hngenetics.org\/wp-content\/uploads\/2024\/09\/\u56fe\u72472-1024x524.png\" alt=\"\" class=\"wp-image-1761\" srcset=\"http:\/\/www.hngenetics.org\/wp-content\/uploads\/2024\/09\/\u56fe\u72472-1024x524.png 1024w, http:\/\/www.hngenetics.org\/wp-content\/uploads\/2024\/09\/\u56fe\u72472-300x154.png 300w, http:\/\/www.hngenetics.org\/wp-content\/uploads\/2024\/09\/\u56fe\u72472-768x393.png 768w, http:\/\/www.hngenetics.org\/wp-content\/uploads\/2024\/09\/\u56fe\u72472.png 1268w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n\n\n\n<p>\u4e2d\u5357\u5927\u5b66\u751f\u547d\u79d1\u5b66\u5b66\u966222\u7ea7\u535a\u58eb\u7814\u7a76\u751f\u9ec4\u60a6\u6208\u548c\u4e0a\u6d77\u79d1\u6280\u5927\u5b66\u514d\u75ab\u5316\u5b66\u7814\u7a76\u6240\u6885\u7ea2\u52a9\u7406\u7814\u7a76\u5458\u4e3a\u672c\u6587\u7684\u5171\u540c\u7b2c\u4e00\u4f5c\u8005\u3002\u4e0a\u6d77\u79d1\u6280\u5927\u5b66\u514d\u75ab\u5316\u5b66\u7814\u7a76\u6240\u5218\u4f73\u7814\u7a76\u5458\u3001\u6885\u7ea2\u52a9\u7406\u7814\u7a76\u5458\u548c\u4e2d\u5357\u5927\u5b66\u751f\u547d\u79d1\u5b66\u5b66\u9662\u674e\u5bb6\u5927\u6559\u6388\u4e3a\u5171\u540c\u901a\u8baf\u4f5c\u8005\u3002<\/p>\n\n\n\n<p>\u6587\u7ae0\u94fe\u63a5\uff1a <a href=\"https:\/\/doi.org\/10.1038\/s41467-024-52193-w\">https:\/\/doi.org\/10.1038\/s41467-024-52193-w<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<div class=\"mh-excerpt\"><p>\u8fd1\u65e5\uff0c\u4e0a\u6d77\u79d1\u6280\u5927\u5b66\u514d\u75ab\u5316\u5b66\u7814\u7a76\u6240\u5218\u4f73\u8bfe\u9898\u7ec4\u4e0e\u4e2d\u5357\u5927 <a class=\"mh-excerpt-more\" href=\"http:\/\/www.hngenetics.org\/?p=1759\" title=\"\u5218\u4f73\/\u674e\u5bb6\u5927\u5408\u4f5c\u53d1\u73b0\u6746\u72b6\u75c5\u6bd2\u611f\u67d3\u54fa\u4e73\u52a8\u7269\u7684\u5bbf\u4e3b\u56e0\u5b50\">[\u8be6\u7ec6]<\/a><\/p>\n<\/div>","protected":false},"author":1,"featured_media":1760,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[17,9],"tags":[],"class_list":["post-1759","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-17","category-9"],"_links":{"self":[{"href":"http:\/\/www.hngenetics.org\/index.php?rest_route=\/wp\/v2\/posts\/1759","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/www.hngenetics.org\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/www.hngenetics.org\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/www.hngenetics.org\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/www.hngenetics.org\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=1759"}],"version-history":[{"count":1,"href":"http:\/\/www.hngenetics.org\/index.php?rest_route=\/wp\/v2\/posts\/1759\/revisions"}],"predecessor-version":[{"id":1762,"href":"http:\/\/www.hngenetics.org\/index.php?rest_route=\/wp\/v2\/posts\/1759\/revisions\/1762"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/www.hngenetics.org\/index.php?rest_route=\/wp\/v2\/media\/1760"}],"wp:attachment":[{"href":"http:\/\/www.hngenetics.org\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=1759"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/www.hngenetics.org\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=1759"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/www.hngenetics.org\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=1759"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}