![\"\"](\"http:\/\/www.hngenetics.org\/wp-content\/uploads\/2017\/12\/d41586-017-08722-3_15313902.jpg\")
<\/p>\n<\/p>\n
\u56fe\u793a\u4e00\u8033\u804b\u7537\u5b69<\/p>\n
\u201c\u8d1d\u591a\u82ac\u5c0f\u9f20\u201d\u7531\u4e8e\u643a\u5e26\u7a81\u53d8Tmc1\u57fa\u56e0\u800c\u8033\u804b\uff0c\u7a81\u53d8Tmc1\u57fa\u56e0\u4e0e\u91ce\u751f\u578b\u57fa\u56e0\u4ec5\u4e00\u4e2a\u78b1\u57fa\u7684\u5dee\u522b\u30022017\u5e7412\u670821\u65e5\uff0c\u7f8e\u56fd\u79d1\u5b66\u5bb6David Liu\u5728Nature\u53d1\u6587\uff0c\u5229\u7528\u8102\u8d28\u4f53\u5c06Cas9\u86cb\u767d\u548csgRNA\u8f6c\u8fd0\u5230\u7a81\u53d8\u5c0f\u9f20\u5185\u8033\u7ec6\u80de\uff0c\u7279\u5f02\u6027\u6572\u9664\u7a81\u53d8\u7684Tmc1\u57fa\u56e0\uff0c\u800c\u4fdd\u7559\u91ce\u751f\u578bTmc1\uff0c\u4ece\u800c\u6cbb\u7597\u8033\u804b\u3002<\/p>\n
\u8be5\u65b9\u6cd5\u89e3\u51b3\u4e86\u57fa\u56e0\u6cbb\u7597\u4e2d\u7684\u51e0\u4e2a\u91cd\u8981\u95ee\u9898\uff1a1\u3001\u5229\u7528\u8102\u8d28\u4f53\u4f5c\u4e3a\u8f7d\u4f53\uff0c\u89c4\u907f\u4e86\u75c5\u6bd2\u8f7d\u4f53\u7684\u53ef\u80fd\u526f\u4f5c\u7528\u30022\u3001\u8f6c\u8fd0\u8fdb\u5165\u7ec6\u80de\u7684\u86cb\u767d\u548cRNA\u505c\u7559\u65f6\u95f4\u6709\u9650\uff0c\u907f\u514d\u8f6c\u8fd0\u57fa\u56e0\u5bfc\u81f4\u7684\u526f\u4f5c\u7528\u30023\u3001\u5229\u7528\u663e\u5fae\u6ce8\u5c04\u5230\u75c5\u7076\uff0c\u907f\u514d\u5bf9\u5176\u4ed6\u7ec6\u80de\u3001\u7ec4\u7ec7\u7684\u5f71\u54cd\u3002<\/p>\n
Nature\u8bc4\u8bba<\/a>\u94fe\u63a5<\/a><\/p>\n \u7814\u7a76\u8bba\u6587\u6458\u8981\uff1aAlthough genetic factors contribute to almost half of all cases of deafness, treatment options for genetic deafness are limited1<\/a>,2<\/a>,3<\/a>,4<\/a>,5<\/a><\/sup>. We developed a genome-editing approach to target a dominantly inherited form of genetic deafness. Here we show that cationic lipid-mediated in vivo<\/i> delivery of Cas9\u2013guide RNA complexes can ameliorate hearing loss in a mouse model of human genetic deafness. We designed and validated, both in vitro<\/i> and in primary fibroblasts, genome editing agents that preferentially disrupt the dominant deafness-associated allele in the Tmc1<\/i> (transmembrane channel-like gene family 1) Beethoven (Bth<\/i>) mouse model, even though the mutant Tmc1<\/i>Bth<\/i><\/sup> allele differs from the wild-type allele at only a single base pair. Injection of Cas9\u2013guide RNA\u2013lipid complexes targeting the Tmc1<\/i>Bth<\/i><\/sup> allele into the cochlea of neonatal Tmc1<\/i>Bth<\/i>\/+<\/sup> mice substantially reduced progressive hearing loss. We observed higher hair cell survival rates and lower auditory brainstem response thresholds in injected ears than in uninjected ears or ears injected with control complexes that targeted an unrelated gene. Enhanced acoustic startle responses were observed among injected compared to uninjected Tmc1<\/i>Bth<\/i>\/+<\/sup> mice. These findings suggest that protein\u2013RNA complex delivery of target gene-disrupting agents in vivo<\/i> is a potential strategy for the treatment of some types of autosomal-dominant hearing loss.<\/p>\n Nature\u6587\u7ae0\u94fe\u63a5<\/a><\/p>\n 10.1038@nature25164<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"